In skyrim what quests can't be completed in one play through. I know about the destroy the dark brotherhood and the civil war quests are their any others?

Um...Well, if you side with the Imperials you can't do the Stormcloaks side either...there's that...
...I guess for the most part, you can do 90% of the quests, limited only by self restraint.

Remember, just because someone gives you a quest, doesn't mean you have to do it.

I am aware of that.