IIRC the only way you can become one again is if you cure yourself of Lycanthropy via becoming a VL, otherwise it still remains impossible.
So far as I heard Aela will restore the beast blood once, no matter how you lost it.
WHAT? Hmm, someone told me you couldn't turn back into one after you got cured of it. Looks like I was wrong then.
That changes when Dawnguard is installed.