Did Jesse really kill Gayle? (Interviews I've seen and read with Vince Gilligan would indicate that he was surprised by any of the murmuring that people thought Jesse moved the gun at the last second, so I'm thinking Gayle is indeed dead)
Will Walter be able to negotiate his survival now that he's in Gus' clutches? (A preview in a television mag mentioned a story arc through the first 3 episodes that covered Walters attempts to screw up enough courage to KILL Gus!)
Will Skylar become part of Walters deception by becoming his Danny?
Will Hank recover from his shooting enough to resume his pursuit of Heisenberg and the Blue Meth?

I think this year we see the end of Jesse. Vince has mentioned that originally the character was meant to die at the end of the first season, but decided to keep him around when there was a positive response from the public. Today I think he's a character that the show can continue without (not that I wouldn't miss him). Killing Gayle is bound to send him into a downward spiral. I can see him going back to the drugs and making some very poor desicions about either Hank or Gus in a wayward attempt to rescue Walt.

Fly, from the third season. There are a lot of people online that dislike that epi, but I think it's brilliant, from Walters obsessive behavior attempting to CONTROL the fly and contamination, to the creepy, weird ending with the blinking red light and the fly trapped inside.

Could the return to the Gayle's death investigation at the end of the episode indicate a greater involvement of police in the season? Could Gus's glasses still contain forensic evidence around the lens' and screws? Sure he washed off after the throat slashing, but didn't pay much attention to his glasses, and blood evidence can be microscopic. Should a murder investigation of Gayle get back to Gus, could he have signed his own conviction by trying to re-assert his dominance over Walt and Jesse?
And another great moment when Jesse and Walt share their thoughts over breakfast. Walt's a genius, but continually demonstrates an inability to look further ahead. Jesse tells him, "now we're all on the same page...." "The same page how?" "The page that says, if I can't kill you I can sure make you wish you were dead." And Walt looks a little surprised. Well duh, Walter, what did you think, Gus was just gonna let things go back to normal now?
Good to see a little bit with Marie and Hank, also, but I really hope he's gonna be able to recover before the season finishes, he just hasn't been the Hank I love to watch since his shooting.

Which I think spoiled some of the surprise of the episode, honestly. I knew right from the opening that the boxcutter Gayle was using would factor in as a murder weapon before the closing credits, simply because I knew the title.

I wonder what's in that folder on Gayle's desk that says, "Lab Notes?" Daa daa duuuuum!

I knew right away that Gus would kill Victor, just from the look on his face when he saw Victor cooking. Also, before the commercials ended there was a warning about graphic content that they don't usually show. So that kind of spoiled that part for me.

I also think Jesse has lost it. I don't know. He was traumatized and then at the end he was perfectly fine. I can't help but shake this feeling that he's finally come to terms that he can't be a good person. I've always considered Jesse to be good at heart, and Walt being a cold hearted sonuvabich. But I don't know what to think anymore.

I honestly can't come up with any predictions that would make sense. I'm sort of pulling these out of thin air, but I think that Skyler is going to start going against Walt, so to speak. And I would bet money that Gus is going to try and get Jesse over to his side. Walt has so much marital problems and self conflict that I think Gus is going to lose faith in him and go for the careless but trustworthy and street smart Jesse, but mostly because he wants Walt dead, obviously.

But, I'm hoping that in the end, Gus dies and Mike will become friends with Walter and Jesse. But I don't know. I just can't read this damn show. So many twists and turns I'm always on edge. I can see the series ending in so many different ways, though. I just can no longer see the series ending with Walter alive. I think he'll die by Jesse or Hank's hand. And I know for a fact that Walt Jr. is going to find out sooner or later, and I have no idea how he'll react to it.

At the beginning of the episode, it was shown to include his notes on the testing of Heisenberg's blue meth. The fact that it was a school folder went with how Gayle was dressed (tshirt and shorts) and his general giddy attitude while setting up the superlab (like a schoolboy in a candy shoppe).... So we know WHAT'S in there, but what will the homicide investigators make of it? Of course this is why I question whether the storyline will include a homicide investigation that finds its way to Gus' door. Should they find Gus as a suspect or the like, checking his glasses would likely reveal traces of Victor's DNA. Will Walt have to kill Gus to avoid his own death, or will the DEA manage to take care of Gus for him?

As for Jesse, I am continuing to predict that he is going to die this season. Killing Gayle has clearly unhinged him, and we all know he's a loose cannon to start with. It wouldn't take much for him to slip back to using drugs, and should he truly conclude that he's a "Bad" person, I could actually see Jesse taking his own life. Or, if they really wanted a dark twist, having Jesse's irrational behavior become a threat to Walter ( a genuine threat like the possibility of Jesse telling Hank or some other authority) and having Walter feel compelled (or even stronghanded by Gus) to kill Jesse himself. Walter is a true rationalizer, and if his own life were threatened he wouldn't have to dig too deep to come up with a reasoning that the most compassionate option for putting Jesse down is that he do it. Right? Walt wouldn't want Gus or Mike to kill Jesse, but under the right circumstances could convince himself to.