After following the http://www.creationkit.com/Bethesda_Tutorial_Optimization I started noticing that were I to leave the roombound (without leaving the cell) the fish were in, depending on where else I went/which directions I looked in, when I returned to the aquarium only a few (or none) of my fish would remain.
I'm pretty sure I'm using all the vanilla spawn scripts but I'll update this post in a sec with the scripts. Any idea how to get my sweet little fishies to stay put/*persist??
http://i101.photobucket.com/albums/m57/will_cuthill/Mzandahrk/ScreenShot843.png.

Thanks.
. That was a screen of the relevant part of my cell. It's fairly sizeable. After I've finished putting all the lighting and other FX in it will definitely be in need of some optimization. It was starting to get a bit laggy for me.