The main piece of evidence toward Vampires still being "alive", I believe, is the fact that Vamperism is a disease. How could a disease survive in Undead tissue? Much less the fact that Vamperism progresses in stages, and can affect a large-scale mutation in the case of a Vampire Lord.
Now I know what you're about to post - Detect Dead. It works on Vampires, and can even be used to detect those in the first stage of the disease. However, is the spell really the best indication that Vampires are infact undead? It's more then possible that the spell isn't sensitive enough to tell the difference between a dead automation and a Vampire due to the massive slowdown of the infectee's metabolic processes, which results in the effective immortality Vampires possess.